FastQCFastQC Report
Tue 22 Jun 2021
H55KCBGXJ_n01_AKM250.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55KCBGXJ_n01_AKM250.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13003194
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC1796881.3818758683443466TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC260570.2003892274467335TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATTC163790.12596135995510024TruSeq Adapter, Index 8 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)