FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_time1p7a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_time1p7a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6794
Sequences flagged as poor quality0
Sequence length151
%GC61

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG320.47100382690609366No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.44156608772446276No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGGGGTTTGCGG260.3826906093612011No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGGGGTTGGCGG140.20606417427141593No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGGGGGTGGGGG140.20606417427141593No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGGGGTTGGGGG120.1766264350897851No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGGGGGTGGCGG120.1766264350897851No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGGGGGTTGCGG90.13246982631733883No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGGGGGTTGGGG90.13246982631733883No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGGCGG80.11775095672652341No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGGG100.0069366856145.01
GTATAAT100.0069366856145.08
AGTATAA100.0069366856145.07
TACACAT3659.309588E-819.8630125
ATACACA3701.07498636E-719.5945934
CACATCT3451.2800847E-618.9130447
TATACAC3551.6838003E-618.3802813
TTATACA3601.9253566E-618.1252
ACACATC3652.1973265E-617.876716
ACATCTC3503.2649383E-516.5714288
CATCTCC3553.6881145E-516.3380289
CTTATAC3553.6881145E-516.3380281
Produced by FastQC (version 0.11.2)