FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7h8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7h8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370666
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13530.3650186421198599No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6340.17104347309977178No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4130.11142106370694911No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA400.00604789454.3753
CGATCTT11350.044.074895
CCGATCT43650.039.5303544
TTCCGAT43650.039.3642582
CTTCCGA43900.039.305241
TCCGATC45450.037.805283
GATCTTT9000.036.256
ATTACAC1854.1673047E-935.270273
CGATCTG18300.034.0710375
GTATAGT1301.6519842E-533.461544
TCTAGGG1801.288663E-732.2222252
GATCTCT4700.029.3085086
ACCGTAC1255.147661E-429.08
ATCTCGC2802.6193447E-1028.4821437
TTAGAGT2303.4751793E-828.3695664
ATCTTTT11050.028.2126717
GCGACCC1855.8031983E-627.4324349
GTATAGA1606.8167385E-527.18751
CGATCTC15800.027.0727835
TATAGTG2156.1071114E-726.9767445
Produced by FastQC (version 0.11.2)