Basic Statistics
Measure | Value |
---|---|
Filename | H55FTAFXY_n01_p7h7.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 216989 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 54 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3419 | 1.5756559088248714 | No Hit |
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 894 | 0.41200245173718486 | No Hit |
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 759 | 0.3497873164077442 | No Hit |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 701 | 0.3230578508587993 | No Hit |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 286 | 0.13180391632755578 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 235 | 0.10830042075865598 | No Hit |
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG | 220 | 0.10138762794427367 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TTAGAGT | 50 | 2.0067216E-4 | 58.0 | 4 |
CTTATGG | 40 | 0.0060445257 | 54.375 | 1 |
GATAGTT | 40 | 0.0060445257 | 54.375 | 7 |
TCATAGA | 55 | 3.2133772E-4 | 52.727276 | 2 |
TAGAGTG | 105 | 6.8728696E-8 | 48.333332 | 5 |
TTATGGG | 45 | 0.009629044 | 48.333332 | 2 |
GTACAGG | 170 | 3.8198777E-11 | 42.647057 | 1 |
ATGTTGC | 90 | 7.463054E-5 | 40.27778 | 1 |
TCTAGCC | 110 | 5.245247E-6 | 39.545456 | 8 |
GTAGGGA | 130 | 3.6805432E-7 | 39.03846 | 3 |
ACACATA | 75 | 0.0014808243 | 38.666668 | 145 |
GTACTTG | 115 | 7.1169034E-6 | 37.826088 | 1 |
TGTTAAG | 440 | 0.0 | 36.250004 | 8 |
CTGGTGT | 180 | 3.1759555E-9 | 36.25 | 4 |
GTCCTGG | 205 | 2.8921932E-10 | 35.365852 | 1 |
ATGGTGT | 165 | 5.979746E-8 | 35.151516 | 4 |
GTTAAGA | 460 | 0.0 | 34.673916 | 9 |
CATGGTG | 210 | 3.7471182E-10 | 34.523808 | 3 |
ATCTAGC | 150 | 1.1284665E-6 | 33.833336 | 7 |
CCATGGT | 195 | 6.9394446E-9 | 33.46154 | 2 |