FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7h7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7h7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216989
Sequences flagged as poor quality0
Sequence length151
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34191.5756559088248714No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8940.41200245173718486No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7590.3497873164077442No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7010.3230578508587993No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA2860.13180391632755578Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2350.10830042075865598No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG2200.10138762794427367No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAGT502.0067216E-458.04
CTTATGG400.006044525754.3751
GATAGTT400.006044525754.3757
TCATAGA553.2133772E-452.7272762
TAGAGTG1056.8728696E-848.3333325
TTATGGG450.00962904448.3333322
GTACAGG1703.8198777E-1142.6470571
ATGTTGC907.463054E-540.277781
TCTAGCC1105.245247E-639.5454568
GTAGGGA1303.6805432E-739.038463
ACACATA750.001480824338.666668145
GTACTTG1157.1169034E-637.8260881
TGTTAAG4400.036.2500048
CTGGTGT1803.1759555E-936.254
GTCCTGG2052.8921932E-1035.3658521
ATGGTGT1655.979746E-835.1515164
GTTAAGA4600.034.6739169
CATGGTG2103.7471182E-1034.5238083
ATCTAGC1501.1284665E-633.8333367
CCATGGT1956.9394446E-933.461542