FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7h6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7h6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205054
Sequences flagged as poor quality0
Sequence length151
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23731.1572561374077073No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6460.3150389653457138No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA4630.22579418104499302Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4290.2092131828689028No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4270.20823783003501517No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA3690.17995259785227308No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC3470.1692237166795088No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC2860.13947545524593521Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA2520.12289445706984502No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT2430.11850536931735055No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG2360.11509163439874373No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA2250.10972719381236162No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA2240.1092395173954178No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2160.10533810605986717No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG2130.10387507680903567No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTTT350.003562486362.1427547
GAGCCTT400.006044095454.374916
ATAACGC553.212988E-452.727189
TCTTGCT604.935869E-448.3332528
CTTCCAA855.3189833E-542.6573871
GATAACG700.00105468441.42858
CCGGACT700.00105468441.42854
CTGCACT1157.115079E-637.8260239
TTCTAGA800.00203043236.2587781
TCCGGAC1001.3876092E-436.249943
ATCTTGT800.00203286536.249947
ATCTTGC1655.977199E-835.151467
GATGGCA1051.8486124E-434.523758
CTTCAGG850.002733679734.125911
TCTGTGC1751.0025542E-733.14288
GGTGGCA1102.4294594E-432.954497
TCTGTAC1102.4294594E-432.954498
CTGTCCA1551.4575144E-632.741889
CCTCCGG900.003617265332.2300261
GCGACCC900.003621589932.2221689