Basic Statistics
Measure | Value |
---|---|
Filename | H55FTAFXY_n01_p7h6.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 205054 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 55 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2373 | 1.1572561374077073 | No Hit |
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 646 | 0.3150389653457138 | No Hit |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 463 | 0.22579418104499302 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 429 | 0.2092131828689028 | No Hit |
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 427 | 0.20823783003501517 | No Hit |
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA | 369 | 0.17995259785227308 | No Hit |
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC | 347 | 0.1692237166795088 | No Hit |
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC | 286 | 0.13947545524593521 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA | 252 | 0.12289445706984502 | No Hit |
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT | 243 | 0.11850536931735055 | No Hit |
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG | 236 | 0.11509163439874373 | No Hit |
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA | 225 | 0.10972719381236162 | No Hit |
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA | 224 | 0.1092395173954178 | No Hit |
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 216 | 0.10533810605986717 | No Hit |
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG | 213 | 0.10387507680903567 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AGCCTTT | 35 | 0.0035624863 | 62.142754 | 7 |
GAGCCTT | 40 | 0.0060440954 | 54.37491 | 6 |
ATAACGC | 55 | 3.212988E-4 | 52.72718 | 9 |
TCTTGCT | 60 | 4.935869E-4 | 48.333252 | 8 |
CTTCCAA | 85 | 5.3189833E-5 | 42.657387 | 1 |
GATAACG | 70 | 0.001054684 | 41.4285 | 8 |
CCGGACT | 70 | 0.001054684 | 41.4285 | 4 |
CTGCACT | 115 | 7.115079E-6 | 37.826023 | 9 |
TTCTAGA | 80 | 0.002030432 | 36.258778 | 1 |
TCCGGAC | 100 | 1.3876092E-4 | 36.24994 | 3 |
ATCTTGT | 80 | 0.002032865 | 36.24994 | 7 |
ATCTTGC | 165 | 5.977199E-8 | 35.15146 | 7 |
GATGGCA | 105 | 1.8486124E-4 | 34.52375 | 8 |
CTTCAGG | 85 | 0.0027336797 | 34.12591 | 1 |
TCTGTGC | 175 | 1.0025542E-7 | 33.1428 | 8 |
GGTGGCA | 110 | 2.4294594E-4 | 32.95449 | 7 |
TCTGTAC | 110 | 2.4294594E-4 | 32.95449 | 8 |
CTGTCCA | 155 | 1.4575144E-6 | 32.74188 | 9 |
CCTCCGG | 90 | 0.0036172653 | 32.230026 | 1 |
GCGACCC | 90 | 0.0036215899 | 32.222168 | 9 |