FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7h5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7h5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171491
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10560.615775754995889No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4070.23733023890466554No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC3980.23208215008367786No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG3940.2297496661632389No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.16210763247050866No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC2690.15685954364952095No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG2650.15452705972908198No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA2590.15102833384842354No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.14053215620644816No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT2400.1399490352263384No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA2150.12537101072359483No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT2130.12420476876337533No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1750.10204617151920509No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAG1551.4551915E-1146.7741933
TCTGAGC951.9115214E-645.7894748
ATCTGAG951.9115214E-645.7894747
CTGCTCT657.3195226E-444.6153839
TCTAGTA1652.7284841E-1143.9393922
ATCTCTT1002.7208425E-643.57
TCTGCTC1201.9606887E-742.2916688
CTACACA907.456491E-540.277784
GCTGGTT1859.640644E-1139.189199
ATCTGGC1859.640644E-1139.189197
CCTAAAT750.00147995738.6666642
TACACAG951.0253309E-438.1578945
ATCTAGT1951.6916601E-1037.179491
TCCTGGG2002.2191671E-1036.252
GTCCTGG1604.551839E-836.2499961
GATCTTT5300.035.566046
AGTAGCT2052.8740033E-1035.3658525
GTAGACG1251.258997E-534.83
TCTTTGC1051.8473338E-434.5238088
CGATCTT9700.034.3814435
Produced by FastQC (version 0.11.2)