FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7h4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7h4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169260
Sequences flagged as poor quality0
Sequence length151
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22291.3169088975540588No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7450.4401512466028595No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4740.28004253810705426No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4670.27590688881011466No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG2690.1589270944109654No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2080.1228878648233487No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG1870.11048091693252983No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG1860.10989010989010989No Hit
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.10989010989010989No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTGC259.3731825E-487.06
ACTGTAC259.3731825E-487.03
ATCCTAC353.4290148E-582.857141
ACAGTGC350.003561387362.1428578
CGTGCTA400.006042239754.3749963
CTACTCT553.2113545E-452.7272729
CTAAGTT553.2113545E-452.7272724
CTACACC553.2113545E-452.7272724
CCTACAC701.6899534E-551.7857133
AGTTCCC604.933368E-448.3333327
GCCTAAG604.933368E-448.3333322
CTAGACG1950.044.6153873
TCTACTC855.3226337E-542.6470578
TAAGTTC700.001054152541.428575
GGCCTAA700.001054152541.428571
CCTAAGT907.456083E-540.277783
ACATTAG750.00147990338.6666687
TCCTACA750.00147990338.6666682
CCTCTCA750.00147990338.6666689
AGACAGA1553.441164E-837.4193576