FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7h3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7h3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123160
Sequences flagged as poor quality0
Sequence length151
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21881.776550828190971No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5570.4522572263721988No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3960.32153296524845726No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3850.31260149399155573No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840.14939915556999026No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA1550.12585254952906788Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1510.12260474179928549No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG1280.1039298473530367No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC301.593869E-596.6666641
TCCTACC300.001931700472.52
CCTATTT300.001931700472.57
CCTAGTA400.00603834454.3752
ATCCGGA400.00603834454.3751
CATAGGT400.00603834454.3755
TACCTAT400.00603834454.3755
GTATATA400.00603834454.3751
CTACTGG450.00961923148.3333321
TCCTGGG1203.5761332E-948.3333322
CCTAAGG450.00961923148.3333323
GCCTAGG450.00961923148.3333321
ATCTTTT5300.045.1415067
TCTTTTT5300.045.1415068
CCTACCT657.3114556E-444.6153833
CTACCTA657.3114556E-444.6153834
GATCTTT5900.044.237296
GTCCTGG1201.9546678E-742.2916681
CTTTTTT5800.041.259
TCTGCAA907.4442294E-540.277788