Basic Statistics
Measure | Value |
---|---|
Filename | H55FTAFXY_n01_p7h3.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 123160 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 54 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2188 | 1.776550828190971 | No Hit |
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 557 | 0.4522572263721988 | No Hit |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 396 | 0.32153296524845726 | No Hit |
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 385 | 0.31260149399155573 | No Hit |
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 184 | 0.14939915556999026 | No Hit |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 155 | 0.12585254952906788 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 151 | 0.12260474179928549 | No Hit |
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG | 128 | 0.1039298473530367 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTCCTAC | 30 | 1.593869E-5 | 96.666664 | 1 |
TCCTACC | 30 | 0.0019317004 | 72.5 | 2 |
CCTATTT | 30 | 0.0019317004 | 72.5 | 7 |
CCTAGTA | 40 | 0.006038344 | 54.375 | 2 |
ATCCGGA | 40 | 0.006038344 | 54.375 | 1 |
CATAGGT | 40 | 0.006038344 | 54.375 | 5 |
TACCTAT | 40 | 0.006038344 | 54.375 | 5 |
GTATATA | 40 | 0.006038344 | 54.375 | 1 |
CTACTGG | 45 | 0.009619231 | 48.333332 | 1 |
TCCTGGG | 120 | 3.5761332E-9 | 48.333332 | 2 |
CCTAAGG | 45 | 0.009619231 | 48.333332 | 3 |
GCCTAGG | 45 | 0.009619231 | 48.333332 | 1 |
ATCTTTT | 530 | 0.0 | 45.141506 | 7 |
TCTTTTT | 530 | 0.0 | 45.141506 | 8 |
CCTACCT | 65 | 7.3114556E-4 | 44.615383 | 3 |
CTACCTA | 65 | 7.3114556E-4 | 44.615383 | 4 |
GATCTTT | 590 | 0.0 | 44.23729 | 6 |
GTCCTGG | 120 | 1.9546678E-7 | 42.291668 | 1 |
CTTTTTT | 580 | 0.0 | 41.25 | 9 |
TCTGCAA | 90 | 7.4442294E-5 | 40.27778 | 8 |