FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7h2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7h2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387500
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC21580.5569032258064516No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA20490.5287741935483872No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA16200.4180645161290322No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT15000.3870967741935484No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14150.36516129032258066No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA13150.3393548387096774No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA12980.3349677419354839No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG11850.30580645161290326No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG11700.30193548387096775No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT11520.29729032258064514No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT11360.29316129032258065No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT10000.25806451612903225No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG9590.24748387096774194No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC8900.2296774193548387No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG8630.22270967741935482No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA7470.1927741935483871No Hit
CTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGG7190.18554838709677418No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG6650.17161290322580647No Hit
ATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGT6500.16774193548387098No Hit
GCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGATAACATCATA5820.15019354838709678No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA5810.14993548387096775No Hit
GTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTC5750.14838709677419354No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT5660.14606451612903226No Hit
GATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAA5650.14580645161290323No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5400.1393548387096774No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT5250.13548387096774195No Hit
GTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCG5170.13341935483870968No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC5050.13032258064516128No Hit
CTGGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGT5050.13032258064516128No Hit
TTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCT4840.1249032258064516No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCT4840.1249032258064516No Hit
GTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGGGGGCA4770.12309677419354839No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG4690.12103225806451613No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG4600.11870967741935484No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCT4590.1184516129032258No Hit
CGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAAC4510.11638709677419354No Hit
GTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATG4500.11612903225806451No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC4450.11483870967741935No Hit
AGCCTACGCTCTGGATACATTAGCATGGGATAACATCATAGGATTTCGAT4440.11458064516129032No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCG4430.11432258064516128No Hit
GATTAGAGGCATTGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAA4360.11251612903225806No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGG4300.11096774193548387No Hit
AACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCT4220.10890322580645162No Hit
GTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGT4210.10864516129032258No Hit
AAGCAAGCCTACGCTCTGGATACATTAGCATGGGATAACATCATAGGATT4170.10761290322580647No Hit
CTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAA4040.10425806451612903No Hit
TCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATGAT4040.10425806451612903No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC3950.10193548387096774No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAAT3890.10038709677419354No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGAC702.3307985E-762.1427462
TTAGAGT3950.055.0631944
TAGAGTG4000.054.37495
GCCATAC450.00962496348.3457221
GTTACTT1203.610694E-948.333253
AAATTAG4850.046.3520851
GTCTAGG1151.4067518E-744.1417471
GAGTGCT5100.041.2254147
ATAAATA4750.039.68414145
CTGGTGT1651.3606041E-939.5453834
GTGCTCA5400.038.9351169
ATCTTGC2057.2759576E-1238.902377
TCTGTCC2450.038.469328
GCGACCC1901.2914825E-1038.1578259
AATTAGA6100.036.8441962
CGATCTT17700.036.454745
ACTCTAC800.002035096536.2499358
TCTAGGG2201.6370905E-1136.2499352
TGTTACT1803.1886884E-936.2499352
ACCTATA7300.035.762591
Produced by FastQC (version 0.11.2)