FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7h1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7h1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109366
Sequences flagged as poor quality0
Sequence length151
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26582.430371413419161No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6050.5531883766435639No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5120.46815280800248704No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4940.45169431084614964No Hit
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1500.13715414296947862No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA1430.13075361629756962Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG1290.11795256295375162No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.11520948009436205No Hit
CGTCTCCCTGTGCAGATCGGAAGAGCACACGTCTCCCTGTGCAGATCGGA1260.11520948009436205Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCCCTGGACAGATCGGAAGAGCACACGTCTCCCTGGACAGATCGGAAGAG1210.11063767532871276Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGAC353.4235756E-582.857142
TCCAGAC955.456968E-1268.684212
TTATAGA350.003558016862.1428575
TTCTAGA400.0060365454.3751
CCCGTTA400.0060365454.3751
CTGGCCT553.2063187E-452.7272769
TGCCACG450.00961636948.3333322
TTCCAGA1303.6582605E-739.038461
TCTGGCC750.001477609338.6666688
TACTGGG951.02289625E-438.1578942
GATCTTT6650.038.1578946
TCTAGGG800.002028706536.252
TCTAGAC2002.1827873E-1036.252
ATCTTTT6400.036.257
CCTGGGT800.002028706536.253
CGATCTT10700.034.5560725
CTAGACG2103.6925485E-1034.5238083
TATAGAT850.002731369634.1176456
CCTATTC850.002731369634.1176453
TCTTTTT6600.034.053038
Produced by FastQC (version 0.11.2)