FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7h10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7h10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737356
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23180.314366466130336No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10330.14009515078198317No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT91450.037.183924
TTCCGAT92750.036.7409132
CTTCCGA94050.036.389721
TCCGATC95950.035.4400143
CGATCTG38100.034.444485
CGATCTT22400.033.986575
CGATCTA17750.029.8188255
TCTAGGG3700.029.3937872
GATCTTT17100.029.2562736
GATCTAG7300.026.8167976
CTAGGGA5750.026.479973
TAGACGT6600.026.3653374
GTCTAGG2351.3281133E-624.684121
TATACTG3251.4824764E-924.5400455
TAGTATA2401.596236E-624.1682262
AGTATAC2101.5535727E-524.1682243
ATCTAGG3001.7056664E-824.1665887
TGTAGAC3352.1063897E-923.8075072
CGATCTC35050.023.16845
GTAGACG4103.8198777E-1122.989293
Produced by FastQC (version 0.11.2)