FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7g9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7g9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303618
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27350.9008029826953607No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11980.39457476170714517No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8470.27896896758426704No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7200.23714009050846788No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4570.15051808522551363No Hit
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3730.12285174133285905No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG3380.11132409804425299No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACT400.00604684454.3754
TAGACTG400.00604684454.3755
TTCTAGA907.470117E-540.2777751
ATAATGC800.00203438636.253
TGTAGAC1655.993206E-835.1515162
GATCTGG8100.034.9074066
TAGACGT6450.034.8449634
CTAGACG4500.033.8333363
ATCTGAG2603.6379788E-1233.461547
ATCTCTG2705.456968E-1232.222227
GTCTAGG1352.1373215E-532.222221
TCTATCC1801.2873716E-732.222228
TAAGCAT950.00472216730.5263184
TCTCTGC2401.5716068E-930.2083348
GTAGACG2401.5716068E-930.2083343
TCTAGGG1703.0026185E-629.8529422
CGATCTG30850.029.6110235
ATCTGGC3550.028.5915497
TCTACAC2054.0235318E-728.2926833
GTACAGG1804.6858204E-628.1944431