FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7g8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7g8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015422
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25880.25486940405072966No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG19990.19686396394799402No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC14570.14348714130676704No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12180.11995012910888281No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG11830.11650328631839767No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT186550.031.7903844
CTTCCGA191700.031.6202031
TTCCGAT189850.031.5433032
CGATCTT44550.030.5948165
TCCGATC195950.030.4873493
ATCTCGC8400.028.4821247
CGATCTG77350.028.400115
GATCTTT22600.028.2300686
GTATCAC4200.027.619033
ATCTGTG6900.027.318827
CACTAGG1358.078182E-426.8544791
TCTGTGC6400.026.054678
CGATCTC61600.024.5981965
TCCTGGG8850.024.5762542
TCTAGAC8900.024.4381872
TAGACGT14650.023.7542484
TCTAGGG5200.023.7019062
CGTATTA3102.4179826E-823.3870812
GATCTGG22700.023.3149626
ACCTATA11350.022.9978451
Produced by FastQC (version 0.11.2)