FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7g7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7g7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359101
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC28220.2076372543320916No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC27110.19947009089096396No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA24830.1826942957145937No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA24800.1824735615675362No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT21870.16091519320492004No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT19660.14465444437168393No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC19180.14112269801876387No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA17930.1319254418913679No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG17280.127142868705122No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT17110.12589204187179612No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT16960.12478837113650862No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT16110.11853423696987936No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC16110.11853423696987936No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT15650.11514964671499764No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG15250.11220652475423092No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA15170.1116179003620776No Hit
ATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCA15120.11125001011698173No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA15030.11058780767580922No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT14470.10646743693073583No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG13620.10021330276410657No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA46400.050.62871
CCGATCT46650.050.198264
TTCCGAT46450.050.1022342
GGGCGAC19100.048.9659467
CGATCTG21350.044.4847565
GGCGACC22000.042.840898
GCGACCC22400.042.3995329
TCCGATC67950.034.7829133
GGGTACC4850.034.3814246
CCCACGC3850.033.896084145
CGATCTT14900.033.573815
CGATCTA9800.031.8112075
GGGGCGA33200.030.1355276
CAGGGGC6100.029.71314
CGATCTC20400.029.4975345
GTTCGAA28350.027.619034145
TAGGACA8700.026.6666534
CTAACGT10250.026.170723
GGTACCT6100.026.147537
TACGAGT16750.025.9701374
Produced by FastQC (version 0.11.2)