FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7g6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7g6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90404
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15651.7311180921198175No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5860.6482014070173886No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4570.5055086058139021No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3550.39268173974602893No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG1520.1681341533560462No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.16149727888146542No Hit
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.15154196716959425No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAT502.000434E-458.03
CTAACGT400.006033167254.3753
TCCTGGG858.8340494E-751.1764682
CTTACAC450.00961101548.3333323
CTGTTCA700.001051544241.428579
GATCTTT5500.040.8636366
CGATCTT7950.039.2138335
ATCTTTT5750.037.8260887
AATCGGA1157.0709575E-637.8260881
CCCTAAC800.002026848536.251
CCCAGAG1001.3814635E-436.252
GTTGCCG800.002026848536.258
TCTTTTT6050.035.9504138
CTCCTGG850.002728874834.1176451
CTTTTTT6550.033.206119
ATCTCGC900.00361093432.222227
ACCTGCC900.00361093432.222228
CCCAAGG1000.006045667529.02
CAGAGCA1000.006045667529.04
CTTCCGA26900.028.2992571