FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7g5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7g5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405561
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9100.2243805494118024No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG8500.20958622747256267No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC7490.18468245220817583No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC5840.14399806687526662No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5580.1375871940349294No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT5500.1356146177763641No Hit
GTACAGGAATATTCACCTGTTGTCCATCGACTACGCCTTTCGGCCTGATC5450.1343817576147608No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA5420.1336420415177988No Hit
GTACTATGCGCTATCGGTCACTGGGAGTATTTAGCCTTGCGCGGTGGTCC5040.12427230428961365No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA5030.12402573225729298No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA4900.12082029583712436No Hit
TAGTAAGGGTCCACGGTGGATGCCCTGGCACTGGAGCCGATGAAGGACGC4760.1173682873846351No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG4630.1141628509644665No Hit
AGATAGTAAGGGTCCACGGTGGATGCCCTGGCACTGGAGCCGATGAAGGA4630.1141628509644665No Hit
GTGATAAAGGAGGTAGGGGTGCAGAGACAGCCAGGAGGTTTGCCTAGAAG4600.11342313486750451No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGTA855.335857E-542.6470572
TACTGTT700.001055897641.428577
TTAGAGT1859.640644E-1139.189194
ATCTTAC1303.6911297E-739.0384647
CGATCTT14800.036.739875
AAATTAG1803.1905074E-936.251
ATCTAGC2103.765308E-1034.5238087
ATCTAGG1301.6523662E-533.461542
AGATAGT2603.6379788E-1233.461541
CTACTGG1102.4336095E-432.9545441
CCGATCT70850.032.4382484
CTTCCGA73150.032.4094351
CCTTATA900.003625707732.222222
TTCCGAT72600.031.856062
CTATAGT1153.1588096E-431.521741
GATCTTT8200.030.9451226
TCCGATC75500.030.6324483
CACTAGG1204.053835E-430.2083341
TAACGTT7550.029.7682114
GTACAAG1504.392341E-529.0000021
Produced by FastQC (version 0.11.2)