FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7g4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7g4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232400
Sequences flagged as poor quality0
Sequence length151
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11810.5081755593803787No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG8300.35714285714285715No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6160.2650602409638554No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC6160.2650602409638554No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG4630.19922547332185883No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG3940.16953528399311532No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC3900.16781411359724613No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA3890.16738382099827884No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3730.16049913941480207No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3250.13984509466437178No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC3230.13898450946643717No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC3210.13812392426850256No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA3030.13037865748709124No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT2750.11833046471600689No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA2730.11746987951807229No Hit
ACCTCCGGCTGAGCCGTTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGA2600.11187607573149741No Hit
ATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGG2550.10972461273666093No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG350.003563060762.1428571
ATACTGC350.003563060762.1428576
TACTGCA400.006045066854.3755
CTACTGC553.213854E-452.7272764
TATATTG604.9371965E-448.3333325
GTATATG450.009629948.3333321
GGCTACA450.009629948.3333321
CTTACAC450.009629948.3333323
TTCTAGA907.464697E-540.277781
CTGTATA907.464697E-540.277784
TGTATAG907.464697E-540.277785
TAACGCA750.001481041938.6666644
ATCTGAC3000.036.257
GATCTTT8250.035.1515126
CTAGACG2700.034.907413
ACACATA1051.8493515E-434.52381145
TCTTACA850.002737677434.1176452
CTTGCCT850.002737677434.1176459
TTCCTAT850.002737677434.1176452
TCTGTCC1501.1289267E-633.8333328