FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7g3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7g3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269747
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22480.8333734944225515No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8640.3203001330876711No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5330.19759255895338965No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5050.1872124620477707No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG4670.17312518767585922No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC3690.13679484850619283No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG3330.1234490096275399No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC3310.12270757413428138No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA3050.11306891272192092No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA3040.11269819497529167Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA3030.1123274772286624No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.10824958201574068No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT2900.10750814652248218No Hit
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2860.10602527553596518No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTAT259.3793584E-486.999776
ATGGTGT1350.069.814644
CATAGGT606.792274E-660.4165153
CCATGGT1650.057.1210672
GTATACT553.2089395E-452.7466961
GCCATGG3100.044.4518551
CATGGTG2150.043.8370973
ACACATA1354.9535447E-737.5925145
ACCTCCG1752.406523E-937.299451
TCCTGGG3900.037.1793942
CAAGGTG2052.8921932E-1035.3657653
AGGTGTT3300.035.1514246
ATAGGTG850.002738486734.117564
GCCAGGT1707.786912E-834.117563
AAGGTGT1956.9521775E-933.4614524
GTCCTGG2705.456968E-1232.2340931
TGTTAAG8100.032.222148
CTCTGCT900.003623616432.2221379
GGTGTTA7850.031.4011946
GTGTTAA8400.031.0713527
Produced by FastQC (version 0.11.2)