FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7g2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7g2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1234307
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC22820.18488107091671682No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC22630.1833417456111No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA20430.165517978914484No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA19120.15490473601786264No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT16890.1368379179572019No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT14390.11658363762013828No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT14340.116178552013397No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA13580.11002125079092964No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC13260.10742870290778551No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG23000.043.1846735
CCGATCT52350.043.0705684
TTCCGAT53150.042.2858772
GGGCGAC16000.041.6873977
CTTCCGA55100.040.5294951
GGCGACC16450.040.106288
GGGTACC4600.039.4020778
GCGACCC17700.039.3219349
GGTACCT5550.032.6575789
TCCGATC70300.032.0731773
GGGGCGA24250.029.5978666
ACTACCG5250.028.9999285
CAGGGGC4750.028.9999264
CCATACT10450.027.7511272
TATATAC6900.027.3187713
TCCCCCC10150.027.142798
CATACTA6200.026.8950942
CGATCTA12950.026.3126775
ATCTAGG2507.8340236E-826.0999342
TAGTATA2859.900759E-925.4385344
Produced by FastQC (version 0.11.2)