FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7g12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7g12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1177279
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC24070.20445450908408289No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC22910.1946012797306331No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA20750.17625388714145074No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA20640.17531952918552016No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT17490.1485629149929626No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT14910.12664797384477258No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA14550.12359007507990884No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT14260.1211267677415464No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT13920.11823875224139732No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC13860.11772910244725336No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG13680.1162001530648215No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT12910.10965964737330743No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG12540.10651680697608637No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT12440.10566739065251311No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG12440.10566739065251311No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA12370.10507279922601184No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA12340.10481797432893988No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAC14550.053.8144047
CTTCCGA39000.052.4274981
CCGATCT39000.052.237154
TTCCGAT40000.050.9312172
CGATCTG18100.047.6657185
GCGACCC18450.044.4037679
GGCGACC18100.043.2596448
TCCGATC53750.038.7116053
CGATCTA8550.035.6140145
GGGGCGA25600.033.417956
GGGTACC4600.031.5217198
CTAGGGG3050.030.901623
TCGCGCG9250.030.567559
GTTCGAA22350.029.843382145
CGATCTC15850.029.7318465
GTCGCGC9650.029.30058
TATAGGT10150.028.5714113
CGATCTT14850.028.316485
TGGGGCG10050.028.1343125
GTACAGG2354.2953616E-827.7683011
Produced by FastQC (version 0.11.2)