FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7g11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7g11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386463
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31250.8086155725127633No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG9910.25642817035524745No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9380.242714050245431No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC7640.19769033516792034No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6460.1671570111498384No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG6030.1560304608720628No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5500.1423163407622463No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5190.1342948742829197No Hit
GTACAGGAATATTCACCTGTTGTCCATCGACTACGCCTTTCGGCCTGATC4370.1130768016601848No Hit
GTGATAAAGGAGGTAGGGGTGCAGAGACAGCCAGGAGGTTTGCCTAGAAG4350.11255928769377664No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC4230.10945420389532762No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA4170.10790166199610313No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG4010.10376155026483777No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGAC752.5476675E-548.333293
TTAGAGT1950.044.6153454
TAGAGTG2350.040.1063465
TACTAAC800.00203508136.249972
TCTATGC1209.548736E-636.2499668
ATCTATG1301.6521764E-533.4615067
CATTAGC2404.3655746E-1133.2291376
CTAACGG1102.4334103E-432.9545174
TCAGACC1102.4334103E-432.9545174
TGGGCCC1102.4334103E-432.9545175
GTCCAAT900.003623216932.2263641
TTCAGGG1851.6392005E-731.3513242
ACATTAG2601.10958354E-1030.6730485
CCTACAG950.004723765430.5262893
TTCCGAG1703.005438E-629.8529152
CTTCCGA77700.029.6756291
ACACATA2202.2566383E-829.659067145
ATCTCTG2451.9645086E-929.591817
CCGATCT76700.029.4914994
TTCCGAT77050.029.451632
Produced by FastQC (version 0.11.2)