FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7g10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7g10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212797
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10420.48966855735748155No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5040.23684544424968398No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2980.1400395682269957No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG2590.12171224218386538No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTAG502.2600943E-887.07
ATACTGT400.00604436654.3756
CCTCCTA604.93625E-448.3333322
CCATACT450.009628789548.3333322
GTCCTAA803.724685E-545.31251
TCTTAGC657.323517E-444.6153878
CCTAACG657.323517E-444.6153872
TATACTA657.323517E-444.6153875
ACAGGAC1151.4055695E-744.1304363
ATACCGT1553.44844E-837.4193576
ATCTATG800.002033019436.257
TCCTACT800.002033019436.254
GATCTTT8800.035.426146
CTAGACA850.002737159434.1176454
GTATAAT850.002737159434.1176451
CTAGGGA2154.820322E-1033.720933
TTCTAGA900.003621863732.222221
CTAACGT900.003621863732.222223
CATAGGG1352.1343332E-532.222222
CTATTGT900.003621863732.222224
Produced by FastQC (version 0.11.2)