FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219842
Sequences flagged as poor quality0
Sequence length151
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56302.5609301225425534No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13100.5958824974299723No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9270.421666469555408No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8000.36389770835418167No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA4280.19468527396948718Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3250.1478334440188863No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2880.13100317500750538No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC2290.10416571901638451Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTGG604.93661E-448.333331
GTATCTG700.001054841141.428571
CTACAGG750.001480866638.6666681
ATCCTGG1001.3879206E-436.251
TCCAGAC1001.3879206E-436.252
GTATTGG1209.529947E-636.2499961
ATCTTGC2850.033.0701757
TCTAGCC1801.2846067E-732.222223
TCTAACC900.003622122632.222228
CTTCTAA900.003622122632.222221
ATCTTTT11600.031.8757
GTGTTAA3250.031.2307687
TGTTAAG3250.031.2307688
TCTTTTT11950.030.9414238
CTAGACG4250.030.7058813
AATCGGA950.00471936130.5263141
GATCTTT12950.030.2316596
ATCTAAG1204.0478553E-430.208331
ATCTAGC1702.997649E-629.852942
TGTAGAC1702.997649E-629.852942
Produced by FastQC (version 0.11.2)