FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190390
Sequences flagged as poor quality0
Sequence length151
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37011.9439046168391199No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10030.5268133830558327No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8080.4243920373969221No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6900.36241399233153004No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA3730.1959136509270445Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.11607752508009873No Hit
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.11555228741005304No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC1960.10294658332895634Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTAGT100.0070863846145.02
TGTGTAC350.003562069762.1428573
ATGCATC400.00604339254.3754
TTAGAGT450.00962724148.3333364
TGCATCG450.00962724148.3333365
TATACTG700.001054484341.428575
GATCTTT9000.037.0555576
CTGCAGG1604.5563866E-836.251
ATCTTTT8050.036.0248457
TGTACTG850.002736438334.1176455
CTTTTTT8650.033.526019
TCTTTTT8650.033.526018
ATCTATG900.003620911632.2222257
CTATACA1153.1525618E-431.521744
CGATCTT16600.030.5722895
CACCGTG1204.045836E-430.2083347
AATCGGA1453.475722E-530.01
ATCTCTG1000.006062276729.07
CGTCTAG4750.027.4736841
CCTAGGG1606.7996094E-527.18752