Basic Statistics
Measure | Value |
---|---|
Filename | H55FTAFXY_n01_p7f8.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 190390 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 54 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3701 | 1.9439046168391199 | No Hit |
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1003 | 0.5268133830558327 | No Hit |
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 808 | 0.4243920373969221 | No Hit |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 690 | 0.36241399233153004 | No Hit |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 373 | 0.1959136509270445 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 221 | 0.11607752508009873 | No Hit |
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 220 | 0.11555228741005304 | No Hit |
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC | 196 | 0.10294658332895634 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TACTAGT | 10 | 0.0070863846 | 145.0 | 2 |
TGTGTAC | 35 | 0.0035620697 | 62.142857 | 3 |
ATGCATC | 40 | 0.006043392 | 54.375 | 4 |
TTAGAGT | 45 | 0.009627241 | 48.333336 | 4 |
TGCATCG | 45 | 0.009627241 | 48.333336 | 5 |
TATACTG | 70 | 0.0010544843 | 41.42857 | 5 |
GATCTTT | 900 | 0.0 | 37.055557 | 6 |
CTGCAGG | 160 | 4.5563866E-8 | 36.25 | 1 |
ATCTTTT | 805 | 0.0 | 36.024845 | 7 |
TGTACTG | 85 | 0.0027364383 | 34.117645 | 5 |
CTTTTTT | 865 | 0.0 | 33.52601 | 9 |
TCTTTTT | 865 | 0.0 | 33.52601 | 8 |
ATCTATG | 90 | 0.0036209116 | 32.222225 | 7 |
CTATACA | 115 | 3.1525618E-4 | 31.52174 | 4 |
CGATCTT | 1660 | 0.0 | 30.572289 | 5 |
CACCGTG | 120 | 4.045836E-4 | 30.208334 | 7 |
AATCGGA | 145 | 3.475722E-5 | 30.0 | 1 |
ATCTCTG | 100 | 0.0060622767 | 29.0 | 7 |
CGTCTAG | 475 | 0.0 | 27.473684 | 1 |
CCTAGGG | 160 | 6.7996094E-5 | 27.1875 | 2 |