FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2092233
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC34250.16370069681531646No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC33690.16102413067760618No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA32260.15418932786166742No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA30850.14745011669350402No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT25910.12383897969298831No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA24970.11934617224754604No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT24210.11571368963208209No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT21880.10457726266625181No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC21510.1028088171824075No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG21030.10051461763579869No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA83700.040.626151
TTCCGAT83900.040.4419172
CCGATCT84950.039.8567054
GGGCGAC22450.036.492167
CGATCTG39300.033.760315
GCGACCC24700.033.755029
GGCGACC25350.033.1755038
TCCGATC111950.030.4383873
CGGGCCG5300.027.35846145
CGATCTA21650.026.7904475
CCCACGC6350.026.259811145
CGATCTT29250.025.5304975
TCCCCCC15700.025.398068
TCGCGCG15550.025.176829
TAGGACA12150.025.0622984
TAGAGTG15650.025.0165425
CTAGGGG5800.025.0005683
GTCGCGC16100.024.3167428
AGGTCGC18150.023.1685716
TTAGGAC12850.023.1328223
Produced by FastQC (version 0.11.2)