FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164147
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22411.3652396936891933No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7470.45507989789639774No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5990.3649168123694006No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5720.34846814136109705No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG3240.1973840520996424No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.13463541825315112No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA2190.13341699817846198Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1900.11574990709546931No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC1860.11331306694609102No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC1760.10722096657264524No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACC350.003561194562.1428574
CTGTCCG400.006041911454.3759
CCTAAGG400.006041911454.3753
TATTAGA400.006041911454.3752
TGTAGAC553.2110664E-452.7272722
TGTACCG553.2110664E-452.7272725
GTACCGG450.00962489348.3333366
GTCTAAG450.00962489348.3333361
CATTACC450.00962489348.3333364
GCCTAGG750.001479771638.6666681
GGTGGCA1854.136382E-935.270277
ATCTATC850.002735342634.117657
ACACATA850.002735342634.11765145
ATCTCAT1102.4273302E-432.9545447
AATCGGA1352.1313761E-532.222221
GATCTTT7300.031.7808236
ATCTCGC2101.4244506E-831.0714287
CGATCTT11700.030.9829045
AGAGTGC950.0047159130.5263186
ATCTGAG1702.9915445E-629.8529427
Produced by FastQC (version 0.11.2)