FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248294
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20190.813148928286628No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6570.26460566908584177No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4400.17720927610010712No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3990.16069659355441535No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG3390.13653169226803707No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA2600.1047145722409724Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG2550.10270083046710755No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC2540.10229808211233457No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGG606.790582E-660.4166681
AATAGTC400.00604555154.3749967
TCTAGGG953.126479E-853.4210552
TTAGAGT1056.8783265E-848.3333324
TACAGGA1105.248312E-639.5454562
TAGAGTG1303.6834172E-739.038465
CCATACT1001.3884387E-436.252
CTAGGGA2451.8189894E-1235.5102043
ATCTGTG2252.0008883E-1135.4444477
GTATCGT1051.8497148E-434.5238081
TAGGGAG7150.034.4755254
GATCTTT8500.034.1176456
TCCTGGG2800.033.6607132
CTAAGCT1102.4309033E-432.9545484
CGATCTT13850.031.4079425
GTACAGG2558.731149E-1131.2745111
CATACTC950.00472052530.5263183
AGAGTGC1702.9997118E-629.852946
ATCTGAC3201.8189894E-1229.4531237
ATTGGGA1504.385563E-529.0000023
Produced by FastQC (version 0.11.2)