FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1285251
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC21200.16494832526876074No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC21130.1644036845721186No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA19860.15452234621875416No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT17770.13826093113329616No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA17760.13818312531949012No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA14740.11468576955007233No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT13100.10192561608588517No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT49650.038.5497974
CTTCCGA50050.038.5389141
TTCCGAT50600.038.11262
GGGCGAC9150.035.655697
CGATCTG22000.033.613595
GGCGACC10400.031.370158
GCGACCC10250.031.121919
TCCGATC65650.029.4858683
CGATCTA11600.026.8749645
GGGTACC3657.2759576E-1225.8218828
GTATAGT4250.025.593181
TCCCCCC8150.024.9079428
CGGGCCG2151.8662933E-523.60462145
GCCCACC9300.022.607496145
CTCCCCC9700.022.4226517
TCGCGCG6250.022.039979
GGTCGCG6800.021.3235027
GTCGCGC6500.021.1922788
CGATCTT18150.021.1707695
TAATACT4901.8189894E-1120.7142584
Produced by FastQC (version 0.11.2)