FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747762
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC15980.21370436047833402No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC15930.2130356985243968No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA14780.19765647358384086No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA13690.18307964298800955No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT11860.15860661547390748No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA10180.13613957382161704No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT9710.12985415145460721No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT8910.11915556019161176No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT8580.11474239129562615No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA8550.11434119412326382No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG8360.1118002786983024No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT8160.10912563088255353No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG8090.10818950414704144No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA8010.10711964502074191No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA7510.10043302548136973No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAC5500.043.499847
CTTCCGA32050.041.851321
TTCCGAT32400.040.7251632
GGCGACC6150.040.0811548
CCGATCT32850.039.946584
GCGACCC6550.037.633459
CGATCTG14400.033.2290425
CAGGGGC2051.1377779E-831.8291514
TCCGATC42900.031.6024463
ACAGTGC1851.6427293E-731.3512368
CCCACGC1851.6427293E-731.351236145
TTGCGAC4100.030.0608634
TAGAGTG4950.029.2928225
GGGTACC1753.7730497E-628.9998938
TAGCACG1000.006073600628.9998934
CTAGGGG2003.2525895E-728.9998933
TCTATAC2752.1282176E-1028.9998933
TATAGTA3052.3646862E-1128.5244872
TAGACTG1804.6980167E-628.194345
CGATCTA7750.028.0644115
Produced by FastQC (version 0.11.2)