FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7f12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7f12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108390
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20041.848879047882646No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4910.4529938186179537No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3320.30630131930989946No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2810.2592490082110896No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC1840.16975735768982378No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG1510.139311744625888No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA1500.1383891502906172No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA1440.13285358427899255No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC1320.12178245225574315No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG1320.12178245225574315No Hit
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.11716948057938924No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT1260.11624688624411847No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA1210.11163391456776456No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.10886613156195221No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG1120.10333056555032752No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTACA100.007072497145.066481
GATTACT100.007072497145.066481
CATGTTA100.007082268144.999544
TCTAGGG554.357571E-879.090662
TAGGGCA406.636533E-572.499774
CATGAGC350.003557973862.1426628
TGTAGAC502.0022494E-457.9998172
GAGTCTT400.00603646854.374836
TTACATG400.00603646854.374833
GTCTAGG450.00959872348.3554951
ATAGGGC450.00961625348.333183
ACGGGAT450.00961625348.333186
CATACAC450.00961625348.333183
GCCCGAA450.00961625348.333182
AGCACGT450.00961625348.333185
GTATAGA700.001052487941.428446
GTAGACG907.438429E-540.277653
AGGTGTT1451.900844E-839.999875
TAGACGT1354.9171285E-737.5924724
TGTTAAG3550.036.7604458
Produced by FastQC (version 0.11.2)