FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7f11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7f11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515846
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30350.5883538885636411No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10200.19773343207081184No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6220.12057862230200485No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTT21500.036.0813985
CCGATCT89050.032.5659754
CTTCCGA91400.032.521881
TTCCGAT90950.032.284222
TCCGATC92650.031.6918493
CTAGACG3700.031.3513533
TAGACGT5300.030.0943414
CGATCTG33500.029.216425
GATCTTT15850.028.3596236
GATCTCG8700.026.6666686
TATCCGT1100.00966732726.3636389
GATCTGG8950.025.9217876
GTCTATA1409.988016E-425.8928552
ATCTCGC3950.025.6962037
ATCTGGC4250.025.5882347
ATCTAGC2951.4244506E-824.5762717
CGATCTC31050.024.049925
CTAGGGA4850.023.9175263
TATGAGT1550.001807307723.3870964
TCTAGAC5400.022.8240742
Produced by FastQC (version 0.11.2)