FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7f10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7f10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800190
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC14080.17595820992514277No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC13890.17358377385370974No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA12310.15383846336495083No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA11910.14883965058298654No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT10570.1320936277634062No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA10150.12684487434234368No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT8470.10584986065809371No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT8470.10584986065809371No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT8330.1041002761844062No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG8320.1039753058648571No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA8150.10185081043252227No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT8110.10135092915432584No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG8100.10122595883477674No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA28950.047.3433571
CCGATCT30900.045.0484664
TTCCGAT30800.044.7239532
GGGCGAC6900.044.130367
GGCGACC7800.040.897378
CGATCTA7150.040.5593725
GCGACCC7750.040.225749
CGATCTG11500.039.0868875
TCCGATC40600.034.4642263
CTATACA3607.2759576E-1226.1805114
GGGGCGA13400.025.9701046
CGATCTC14550.025.9106085
TAGGACA4001.8189894E-1225.3749564
TCCTGGG3151.033186E-925.3174172
GTCGCGC4900.025.1530198
ATAATAC2601.1441625E-725.0961113
GCTATAA1751.2533044E-424.8633161
TCTTCCG20700.024.5168675
TGGGGCG5650.024.3804875
CATACAC2751.967619E-723.7272323
Produced by FastQC (version 0.11.2)