FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3751367
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG156730.41779436669352793No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT139330.3714112748765983No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG110350.29415943574702236No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT109060.2907206892847327No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC103660.27632593665189253No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC101080.26944844372731325No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC100040.2666761209980255No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCG95590.25481377855059234No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA95300.25404072702031016No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA93750.24990889987569864No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC93190.24841611071377448No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTT90800.24204509982627664No Hit
GATTAGAGGCATTGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAA88970.23716687810070303No Hit
TCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGT88080.2347944096112164No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA87580.23346156214521266No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC85460.22781028888935687No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT83810.22341189225154456No Hit
GTCAAAGAGTGCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCCGGCGA83660.22301203801174344No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG83580.22279878241718287No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC81620.21757402035044826No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGG81160.21634780068172482No Hit
GGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTG80770.21530817965824192No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTA76920.2050452541700132No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC74700.19912741142095666No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCG74660.19902078362367637No Hit
GTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGT73580.19614183309710834No Hit
TTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCT72560.19342282426646074No Hit
TTTCAAGACGGGTCGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGC72470.19318291172258006No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCT71840.19150352391541536No Hit
GTCTTGAAACACGGACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGTG71540.19070381543581313No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT69620.18558568116635882No Hit
CCTCTAATCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTA68960.1838263225112339No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCT68130.18161379571766773No Hit
CTCCACAAGCGTATCAAAAGCCCGGGCTTAGGCCGCCACCGTAATCCGCG67360.17956121062002198No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCT66870.17825502010333832No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGAC65490.17457636109716804No Hit
ATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAATGCCTCTAA64990.17324351363116433No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAAT64670.17239049125292194No Hit
TTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCC63790.1700446797127554No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT63770.16999136581411523No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT63710.16983142411819477No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC63700.1698047671688747No Hit
GGCTTAGGCCGCCACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCG63030.16801875156442972No Hit
GGCTTTACCCCGCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGC60460.16116791558917057No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA58100.15487687554963298No Hit
TATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAA57130.15229115146558575No Hit
TCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGA56670.1510649317968623No Hit
TAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCT56020.14933223009105748No Hit
ATACTGACGTGCAAATCGTTCGTCTGACTTGGGTATAGGGGCGAAAGACT54710.1458401697301277No Hit
CCGTTAGGCGCGTGCTGCAGACCACGATCACGGCAGCGACGTCTCCACAA54620.14560025718624706No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTCCAAGGCTAAAT54510.14530703074372622No Hit
CCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCGAC54080.14416078192296303No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT54040.1440541541256827No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCG53940.14378758463248198No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA52970.14120186054843475No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATT51840.13818962527526632No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG51190.13645692356946149No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA50870.1356039011912191No Hit
CATGTCAGACTCCTTGGTCCGTGTTTCAAGACGGGTCGAATGGGGAGCCC50850.13555058729257896No Hit
CTTCTCAGAAGATCAAGGTCGGTCGGCGGTGCACCCGCGAGGGATCCCGC50590.134857506610257No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGT50550.1347508788129767No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG50490.13459093711705625No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT50190.13379122863745402No Hit
TCCATGACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGTT49980.13323143270173246No Hit
AACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAA49180.1310988767561265No Hit
CGTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCA49100.1308856211615659No Hit
ATGGTGAACTATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAA49000.13061905166836515No Hit
GTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGG48080.1281666123309183No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT47800.1274202177499562No Hit
GATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGT47360.12624731197987293No Hit
CCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTT46890.12499443536182944No Hit
AGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGC46420.12374155874378594No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA46400.12368824484514578No Hit
GTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAA44680.11910324956209295No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCA44470.11854345362637139No Hit
CCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGG44440.11846348277841118No Hit
GTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGA44410.11838351193045095No Hit
ACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCGGCGGACCGGATTG44120.11761046040016879No Hit
GTTCACCATCTTTCGGGTCCCGACAGGCATGCTCACACTCGAACCCTTCT43580.11617098513688477No Hit
TGTCAGACTCCTTGGTCCGTGTTTCAAGACGGGTCGAATGGGGAGCCCAC43380.11563784615048328No Hit
ATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCG43220.11521133496136207No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG43210.115184678012042No Hit
GGCATAGTTCACCATCTTTCGGGTCCCGACAGGCATGCTCACACTCGAAC43110.11491810851884127No Hit
GAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG43090.1148647946202011No Hit
AACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGG42940.1144649403804No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC42350.1128921803705156No Hit
ACCTTGATCTTCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCC41980.11190587324567286No Hit
ACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGT41740.11126610646199106No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG41660.11105285086743047No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAG41310.11011985764122784No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA41150.10969334645210665No Hit
TTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCC40990.10926683526298547No Hit
GCGCTTAAGCGCGCGACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCT40570.10814724339154233No Hit
GATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTTTGCAG40490.10793398779698174No Hit
CCCTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGT39360.10492175252381332No Hit
TCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAG39100.10422867184149139No Hit
GGATAGCTGGAGCTCGGACGCGAGTTCTATCGGGTAAAGCCAATGATTAG38980.10390878844965049No Hit
GTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATC38680.10310907997004826No Hit
CCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTC38350.1022294006424858No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG38230.1019095172506449No Hit
CTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGG38170.10174957555472446No Hit
TCTCGATTCCGTGTGACGGCTCAACAAAGCAGCCGACACGTCCTACCTAT38020.10134972131492334No Hit
GAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGC37850.10089655317648207No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA37840.100869896227162No Hit
TCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGC37550.10009684469687982No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT143850.053.323582
CTTCCGA145850.053.189591
CCGATCT151900.050.6885954
GGGCGAC71450.050.2274067
CGATCTG60050.049.501045
GGCGACC74200.048.5612958
GCTGTGC5500.044.818158
GCGACCC81900.044.79249
CAGGGGC15100.043.6926084
CGAGGGG12100.041.94212145
ACACATA10150.041.428543145
CGATCTC51150.039.829445
CGATCTT35150.039.1896865
GATCTCG14700.038.963086
CGATCTA35150.037.7458535
CCTATTA4050.037.592567145
CGGGCCG17250.037.405773145
TCCGATC210800.036.8695373
CTAGGGG10100.036.6093753
GATCTGA18250.035.3566136
Produced by FastQC (version 0.11.2)