FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429229
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32320.7529780140670831No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG11970.2788721172148201No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10500.24462466422352636No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG10220.23810133984423235No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC7790.18148820326678766No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7450.1735670236633592No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC6350.14793967788756118No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5540.1290686323617463No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA5330.12417613907727577No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA5200.12114745275831783No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA5160.12021554927556152Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG4710.10973163509455325No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC4570.10646997290490623No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCT4480.10437319006870457No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC4450.10367426245663737No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAGT1700.046.9117244
TAGAGTG1602.0008883E-1145.312465
GTACAAG1105.2530995E-639.550031
CCTTTTA750.001482447638.6666372
AAATTAG1951.6916601E-1037.183791
CTGTCCG1051.8519667E-434.5237859
GATCTTT13300.034.342086
TCTTTTT11450.034.1921128
TACAGGA2404.3655746E-1133.229142
GTCTAGG1102.4321917E-432.958361
CGATCTT23850.032.8301625
ATAAATA2455.4569682E-1132.550995145
TATAAGC3650.031.7807983
TCCTGGG4300.030.3488122
TATAGGT4800.030.208313
CTTTTTT13050.029.9999779
AATTAGA2451.9645086E-929.5918122
GGCCCTA1000.006070669728.9999797
TAAGCAA4050.028.641955
ATCTTTT14100.028.2801197
Produced by FastQC (version 0.11.2)