FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550099
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC10220.18578474056488015No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC9560.17378690017615012No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA8790.15978941972263175No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA8750.15906227788089053No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT6590.11979661842686498No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT6370.1157973382972883No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG6210.11288877093032346No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA6080.1105255599446645No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT5950.10816234895900556No Hit
GCCTAGGCGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGC5640.10252699968551116No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT5610.10198164330420526No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATAC1553.4655386E-837.4193573
CCGATCT37100.036.347714
CGATCTG15250.035.655745
TTCCGAT38200.035.4908372
TAGAGTG4100.035.3658525
CTTCCGA40100.034.351621
TAGACGG850.002740917534.117655
GTATAAG1301.6534332E-533.461541
ACTACCG3700.033.3108145
CGATCTT10200.031.9852925
CATACTA3650.031.7808232
GTCTATA1153.1602633E-431.521741
CTCTAGG1153.1602633E-431.521741
GTCTACG5550.030.0450421
TTAGAGT4600.029.9456544
TCCGATC46150.029.6912253
TAGTATG3450.029.420292
ATACTAC4450.029.3258423
CGAGTCG5500.029.06
GTATCAT1504.3951542E-529.01
Produced by FastQC (version 0.11.2)