FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015597
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC17160.16896465822565446No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC16230.1598074826924459No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA15920.15675509084804307No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA15750.15508119854627378No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT13220.13016974252582472No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA11230.11057535616981932No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT10610.10447057248101363No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT10260.10102432362442976No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA42350.039.20691
CCGATCT42400.038.4728554
TTCCGAT43050.038.2287752
CGATCTG19000.036.2499775
TAGAGTG7000.034.178555
GTATATA4150.033.196021
TCTAGGG2653.6379788E-1232.830172
CTAGGGG2301.0022632E-931.5217173
TCCGATC55350.030.126453
TTAGAGT8050.029.7204764
GGGCGAC6750.028.999987
TATACGG1855.8162823E-627.4324155
ATGCCGG5450.026.605486145
GTGCTCA8000.026.2812339
TATATAC5000.026.0999833
CGGGCCG2507.831113E-826.099983145
GGCGACC7550.025.9271348
GCGACCC7950.025.5345739
ATTATAC3709.094947E-1225.4729563
GTCTAGG2901.1894372E-825.0024431
Produced by FastQC (version 0.11.2)