FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852053
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA24200.284019890781442No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC23220.2725182588407059No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA20700.24294263385024173No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC18970.22263873256710554No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT15920.18684283724134532No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT15440.18120938486220928No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT14900.17487175093568122No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA12960.15210321423667306No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG12850.15081221473312106No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT12420.14576558031014503No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT11800.13848903765376097No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA11750.13790221969760097No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA11460.13449867555187295No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG11340.13309031245708894No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT11090.1301562226762889No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA10670.12522695184454488No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA10570.12405331593222488No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA10140.11900668150924884No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG9270.10879604907206478No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC9090.10668350442988876No Hit
CCTATGATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGA8910.10457095978771273No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT41950.048.0452162
CTTCCGA41750.047.9393161
CCGATCT43200.046.3193664
CGATCTG17550.042.96295
GGGCGAC6050.040.7437367
ACGGACG2350.040.106316145
GCGACCC6700.036.7909859
CGATCTA10450.035.382725
TCCGATC57900.035.1856083
GGCGACC7500.032.8666158
TGCAGGG3100.030.4031772
GTATATA3400.029.8599031
GTATTGG2701.70985E-1029.5439241
CCTATAA18300.029.3237741
CGATCTT12700.027.9723955
CGATCTC19250.027.8700855
TATAAGC20350.027.7886543
CTATAAG19700.026.8654382
TAGAGTG7600.026.7104825
ACCATAC8750.025.6917041
Produced by FastQC (version 0.11.2)