FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371185
Sequences flagged as poor quality0
Sequence length151
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20940.5641391758826461No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9110.2454301763271684No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5940.1600280183735873No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5760.1551786844834786No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5190.13982246049813435No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA5040.13578134892304378No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA4850.1306626075945957No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG4420.11907808774600268No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA4210.11342053154087585Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA4180.11261230922585772No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACC604.941036E-448.3333323
TGTAGAC1551.4551915E-1146.7741932
TAGCACT657.3305983E-444.6153834
TACACTG1651.3606041E-939.5454525
CTATACG800.002034968536.254
TCTAACG800.002034968536.252
CTAAACC1051.851477E-434.5238083
CTACACT1051.851477E-434.5238084
CTAGGGA4700.033.9361723
CTAGACA2206.202754E-1032.9545444
GATCTTT13550.032.103326
CTCTAGG1153.158297E-431.521741
AGACAGT1402.7420627E-531.0714286
TCTAGGG2351.2569217E-930.8510652
TCTAGAC4850.029.8969082
CATAGGT1000.006069602429.03
GCCATGG3800.028.618421
CTAGACG5150.028.1553423
ATCTTTT13650.028.150187
CGATCTT24700.027.8846155
Produced by FastQC (version 0.11.2)