FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361143
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22420.6208067164530394No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11800.32674037708054704No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7050.19521353037439462No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG6030.16696987066065241No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA4620.1279271645857735Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4380.12128159759430474No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGGT855.3347194E-542.647023
CCATAGG1157.1300983E-637.826052
TAGGACT1102.4330968E-432.9545144
CTCTAGG900.003622745432.226651
CATAGGG2051.1343218E-831.8292372
GTACCGT1153.1581477E-431.5217086
TGTAGAC1851.6387094E-731.351322
ATCTGAC4000.030.812477
TAACGTT950.00472336130.5262854
CTTAGGG1902.070392E-730.5262852
CTAATGA1204.0529863E-430.2083033
GCCATAG1453.4810153E-530.0041261
GCTTAGG1453.4810153E-530.0041261
ATAGGTG1453.4843084E-529.9999734
GGCTAGG1504.386907E-529.0039861
TGTACTC1255.1474286E-428.9999755
TGTACCG1000.006069415728.9999715
ATATTAG1804.684036E-628.198321
CGTATGG1555.4908705E-528.06449145
GATCTTT16600.027.951786
Produced by FastQC (version 0.11.2)