FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512592
Sequences flagged as poor quality0
Sequence length151
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29600.5774573149795549No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG12150.23703062084464835No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10400.20289040796578955No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC9060.17674875924712052No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA8810.1718715859787121Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7000.13656085151543526No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6650.12973280893966352No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC6080.11861285388769235No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA5300.10339607329025813No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG5290.10320098635952181No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC5280.10300589942878546No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGA2350.040.106382
CAGGAAT2800.036.254
AGGAATA2750.034.2727245
GTACAGG4150.033.1927721
TAGAGTG1551.4631623E-632.7419365
ATCTGTG5000.031.9000027
TTAGAGT1402.744086E-531.0714284
CTGCTAA950.004725169430.5263181
CTCTATG950.004725169430.5263189
CCTATAG1204.0553143E-430.2083343
ATAACAT3201.8189894E-1229.4531253
CTGACCC1255.150377E-429.0000029
TCTAGAC6500.029.02
ATCATAG3301.8189894E-1228.5606088
GGAATAT3052.3646862E-1128.5245916
ACAGGAA3052.3646862E-1128.5245913
CATCATA3351.8189894E-1228.1343297
CTATGCT1050.007705004427.6190474
CTGTCGT1050.007705004427.6190479
TCTGTGC4500.027.388898
Produced by FastQC (version 0.11.2)