FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7e11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7e11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250118
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28951.1574536818621612No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5460.21829696383307082No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4910.19630734293413507No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4020.1607241382067664No Hit
CCTCTAAAAGAGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCGCTAT3040.1215426318777537No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTG651.0912618E-555.769237
TAAGACC400.006045602754.3754
AACACTA400.006045602754.3755
ACACATA1353.6379788E-1253.703705145
ATCTGTG2000.050.757
CCTAATG1002.7258848E-643.52
ATCTGCC3100.042.096777
CTAATGG1053.8125945E-641.428573
TAATGGT1209.535201E-636.254
CTTCCGA46950.035.053251
TCCGATC46450.034.9623263
TTCCGAT46850.034.818572
CCGATCT46350.034.56854
TCTGTGC2103.7471182E-1034.5238088
CGATCTG17950.034.331485
GTCCTGG1301.6498449E-533.4615361
TTAGGGA1102.430955E-432.9545483
TATACTG1102.430955E-432.9545482
CGATCTT10800.032.893525
CTCTTGG1551.4591951E-632.7419361
Produced by FastQC (version 0.11.2)