FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174855
Sequences flagged as poor quality0
Sequence length151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5340.3053958994595522No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.18872780303680192No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA2440.13954419376054444Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCGT400.00604257254.3756
ACTGAAT450.009625941548.3333328
TCCTACA604.9338193E-448.3333322
CTACTGG803.721944E-545.31251
CATAGAT1209.518792E-636.257
TCCGTTG1001.3867594E-436.258
GTTCAGG800.002032030636.251
ACATAGA800.002032030636.256
ATACATC800.002032030636.256
TCTGCTC1604.5527486E-836.258
CTAATCC1051.8474832E-434.5238083
TATACCC1102.4279779E-432.9545444
ATCTAGC1352.132171E-532.222227
TCTAGCA900.003620111832.222228
TCCTGGG2558.731149E-1131.274512
ATCTTTT3750.030.9333347
CCTACAC950.00471674830.5263183
GATCTGG4800.030.2083346
TATGGGA1702.9930252E-629.852943
TACAGGG1952.5865302E-729.743592
Produced by FastQC (version 0.11.2)