FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166325
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2960.17796482789718926No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG2400.1442958064031264No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC2230.13407485344957162No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG2080.1250563655493762No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.11363294754246205No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC1890.11363294754246205No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTGAG902.035631E-856.388897
ATCTGAC1800.052.361117
TCTGACC1152.4629117E-950.4347848
CTGGACT450.00962511948.3333324
GATCTGA3400.046.9117666
CTACCCG803.7211525E-545.3125049
GATCTAC2400.045.31256
CTCCGGC1307.2868716E-944.6153833
CTAGGGA1652.7284841E-1143.9393923
TCCGGCT1002.7203478E-643.54
TTCCGAT29000.040.52
CTTCCGA29250.040.401711
CGATCTG12250.040.2448965
CCGATCT29250.040.153854
TCCGATC30200.038.8907283
CTGAGCC1502.577326E-838.6666649
TATATAC750.001479829238.6666643
TCTGTCA800.002031744736.2500048
ATCTACG1001.3864676E-436.257
GATCTTT3250.035.6923076