H55FTAFXY_n01_p7d6.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H55FTAFXY_n01_p7d6.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	127291
Sequences flagged as poor quality	0
Sequence length	151
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG	613	0.481573716916357	No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC	431	0.33859424468344185	No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC	346	0.27181811754169577	No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG	345	0.27103251604591055	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	309	0.2427508621976416	No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA	264	0.20739879488730545	No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA	246	0.19325796796317102	No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA	240	0.1885443589884595	No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT	238	0.18697315599688902	No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG	234	0.18383075001374802	No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC	210	0.16497631411490207	No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT	209	0.16419071261911683	No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC	204	0.1602627051401906	No Hit
ACCTCCGGCTGAGCCGTTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGA	186	0.14612187821605613	No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	185	0.14533627672027088	No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA	170	0.13355225428349216	No Hit
ATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAAC	154	0.1209826303509282	No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG	152	0.11941142735935768	No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT	152	0.11941142735935768	No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA	150	0.1178402243677872	No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT	149	0.11705462287200194	No Hit
ATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGG	145	0.11391221688886095	No Hit
CTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAA	145	0.11391221688886095	No Hit
GTACAGGAATATTCACCTGTTGTCCATCGACTACGCCTTTCGGCCTGATC	143	0.11234101389729047	No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCA	141	0.11076981090571997	No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCT	140	0.10998420940993472	No Hit
CTGCAAAACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATC	140	0.10998420940993472	No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCG	140	0.10998420940993472	No Hit
ATTTTAACCGGATCCCCTTTCGAAGTTCGCGCTTAAGCGCTATCAGACGG	137	0.10762740492257898	No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC	137	0.10762740492257898	No Hit
GCGCTTAAGCGCGCGACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCT	135	0.10605620193100847	No Hit
ATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGCGCGAACTTC	134	0.10527060043522324	No Hit
GATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAA	132	0.10369939744365272	No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG	131	0.10291379594786748	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCTAG	35	0.003559357	62.142853	1
ATAAGCT	35	0.003559357	62.142853	4
ATTGCAC	35	0.003559357	62.142853	3
TAAGCTT	35	0.003559357	62.142853	5
ATCTGTG	35	0.003559357	62.142853	7
GATAAGC	40	0.0060388055	54.375	3
GCTCTGT	40	0.0060388055	54.375	1
ATAAGCA	95	3.11029E-8	53.421055	4
ACACATA	55	3.2083192E-4	52.727276	145
TACAGGA	110	1.6552804E-9	52.727276	2
CTATAAG	100	4.659887E-8	50.75	2
TATAAGC	100	4.659887E-8	50.75	3
AGGAATA	115	2.4556357E-9	50.434784	5
TAGAGTG	60	4.928718E-4	48.333332	5
TCTTGTC	60	4.928718E-4	48.333332	8
TACTGGG	90	1.314409E-6	48.333332	2
TACTGAG	45	0.009619963	48.333332	2
CAGGAAT	120	3.5779522E-9	48.333332	4
CCTATAA	110	9.867654E-8	46.136368	1
ATCTGGC	110	9.867654E-8	46.136368	7

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content