FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785974
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC14180.180413092545046No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC13800.175578327018451No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA13410.17061633082010347No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA12180.15496695819454587No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT10770.13702743347744326No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT9420.11985129279085567No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT9170.11667052599704315No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT8920.11348975920323064No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG8610.10954560837890312No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA8460.1076371483026156No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT8310.10572868822632811No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT8090.10292961344777309No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA8020.10203899874550557No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAC6250.061.4799967
CTTCCGA20150.048.9330061
TTCCGAT21200.047.1933942
GGCGACC8150.046.257678
CCGATCT21750.045.3333324
GCGACCC8750.043.9142889
CTAGGGG1505.366019E-1043.53
CGATCTG10100.038.044555
TCCGATC31150.031.653293
GGGGCGA14400.031.2152796
TATAAGG2651.382432E-1030.0943372
CAGTATA2202.2630047E-829.6590922
TATACAT3950.029.3670885
CAGGGGC2252.8154318E-829.0000024
CTGGGCG1255.1528483E-429.09
TGGGGCG5550.028.7387395
TCTAGAC2405.2687028E-827.18753
ATATAAC3005.857146E-1026.5833323
GTTATAC1958.751376E-626.0256391
CGATCTA6200.025.7258055
Produced by FastQC (version 0.11.2)