FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99893
Sequences flagged as poor quality0
Sequence length151
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2830.28330313435375853No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG2170.21723243870941908No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG1770.17718959286436486No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1670.16717888140310133No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC1630.1631745968185959No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC1550.15516602764958506No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA1240.12413282211966804No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA1050.10511247034326729No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA1040.10411139919714095No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTAGA300.001930625272.52
CATAGAC400.00603500954.3752
CTCTCCA400.00603500954.3759
CATGGTG953.101195E-853.4210553
CTCAACC553.2049653E-452.727276145
TCTCTCC553.2049653E-452.7272768
CTAAGGA604.923581E-448.3333322
GTGTGTA901.3115368E-648.3333329
CCTCCTA450.0096139448.3333322
ATGGTGT1056.822847E-848.3333324
AGTGTGT803.7104015E-545.31258
ATCTTAC657.304778E-444.6153837
CCATGGT1002.7093738E-643.52
TCTAGGG855.306891E-542.647062
CCTACTC700.001052073941.428573
TACAGGG907.434086E-540.277782
TCTAGAC750.001476993438.6666643
TCCTACT750.001476993438.6666642
GGAGTGT951.02225735E-438.1578946
CTAGTAG1001.3825003E-436.253