FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770595
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC17570.22800563201162738No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC15670.20334935991019928No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA14580.18920444591516944No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA14460.1876472076771845No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT11680.15157118849720022No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT10330.1340522583198697No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA9870.12808284507426082No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT9830.12756376566159913No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT8960.11627378843620838No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT8890.11536539946405051No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC8810.11432724063872722No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG8760.11367839137290017No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT8500.11030437519059946No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA8090.10498381121081762No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT8060.10459450165132139No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT8040.10433496194499055No Hit
ATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCA7810.1013502553221861No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA7760.10070140605635905No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT23900.049.4455642
CTTCCGA24600.047.160551
CCGATCT25450.047.0038874
GGGCGAC9750.043.8717587
GGCGACC10800.039.606458
CGATCTG12650.038.3991745
GCGACCC11350.038.3259549
TAGAGTG5800.036.2499665
TCTAGGG2103.783498E-1034.523782
GTCTAGG1501.1323009E-633.8376961
TCCGATC35950.033.477023
GGGTACC2257.912604E-1032.222196
TGGGGCG6350.031.9684755
CAGGGGC2507.094059E-1131.8999714
CGATCTT8850.031.1299135
CGTAGGG950.004726649730.5262892
GGGGCGA15250.030.42626
GTATAAC2151.806984E-830.3527491
GTTCGAA13350.029.868887145
CGATCTC10100.028.7128475
Produced by FastQC (version 0.11.2)