FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128449
Sequences flagged as poor quality0
Sequence length151
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4750.36979657295891755No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2980.23199869208791038No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG1840.14324751457777016No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA1540.11989194154878591Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGTA300.001931908972.499812
TACACCA451.18993026E-464.444275
GTACTGG1150.063.0678671
GTCTGTA350.003559467862.14269145
TACTGGG1307.2650437E-944.6152652
CTACACC855.315508E-542.6469424
CTAGGAC700.001053214841.428463
CCTGTAC750.001478589838.6665653
CTGTCCA1001.3847287E-436.24999
CTGCCTT800.002030049536.24999
CGATCTT7500.035.766575
GATCTTT4750.035.1051676
TAGGACG1352.1278232E-532.2221374
ACACCTT900.00361660432.2221346
ACGGTAT900.00361660432.2221348
ATCTGTG2101.4193574E-831.0713447
GACGGTA950.004712189530.5262347
TCTGCCT1204.038634E-430.2082548
TCTGTGC1453.4670644E-529.999928
GACTAGG1000.006043584629.011221
Produced by FastQC (version 0.11.2)