FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7d12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7d12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71480
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8831.2353105763850027No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3030.42389479574706207No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1720.24062674874090656No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1510.21124790151091213No Hit
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770.10772243984331281No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG750.10492445439283715No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTTAA100.007062584145.100141
CTTATCG100.007077391144.99863
CTCCTTA350.003543253362.1857761
GATTAAG350.003543253362.1857761
TAGCGGA350.003553103262.1422586
ATCTCGC400.006011548554.412551
CCATGGT400.006011548554.412552
CTTTAAT400.006011548554.412552
CTGTCCT450.00960319348.3328639
TATCTCG450.00960319348.332863145
CTTAGCG450.00960319348.3328634
ATCTGGC450.00960319348.3328637
TATATGT803.6999416E-545.312064
TGCCAGG657.26598E-444.6461942
AGGTGTT657.2911126E-444.6149525
CGATCTG4150.040.1803325
GATCTGG1451.8853825E-839.9996156
CTAGGGA951.01939615E-438.1575284
ATATATG800.002024102736.249653
GTATTTA1051.8291394E-434.5476531