FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7d10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7d10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130176
Sequences flagged as poor quality0
Sequence length151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3500.2688667649950836No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA1900.14595624385447392Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.13904252704031464No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC1740.13366519174041297No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG1590.12214233038348084No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC1380.10601032448377581No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT353.426031E-582.857144
GTTTATA400.006039113754.375145
ACGACAG400.006039113754.3755
AACCATG450.00962045148.3333327
GTCTATG450.00962045148.3333322
GCCTGAA450.00962045148.3333322
GACTGTG450.00962045148.3333327
TATACTG604.9291376E-448.333335
TATAGGT951.9079453E-645.7894743
CTATGAT657.312994E-444.6153874
GGTCTAT657.312994E-444.6153871
ATAGGTC855.315826E-542.6470574
TCCTGGG1105.2290598E-639.5454562
GGTGGCA1604.537287E-836.259
TCTATAC800.002030124736.253
TAGGTCG1051.8448936E-434.5238085
CTAGGGA1905.349648E-934.3421063
CTAGAAG850.002733272734.1176453
TAGAAGC850.002733272734.1176454
GCCTAGA850.002733272734.1176451